Breakthrough in enamel regrowth

0
1971

Smiling dentist and patientTwo research breakthroughs at the University of Southern California (USC) are promising the possibility of a substance that can repair and regrow enamel. Janet Moradian-Oldak, a dentistry professor at the Herman Ostrow School of Dentistry of USC, says it could lead to products like an enamel regrowing gel that lessens or eliminates pain associated with dental caries.

It’s early days. The US Food and Drug Administration has not yet approved any type of enamel regrowing gel. USC is in pre-clinical trials. Prof. Moradian-Oldak said one day people may be able to use an overnight mouth guard or teeth strips saturated with hydrogel to regrow enamel-like substances and reduce teeth sensitivity.

In October, in collaboration with lead author Sauma Prajapati of USC and other colleagues, Prof. Moradian-Oldak published a study in the Biomaterials journal saying matrix metalloproteinase-20, an enzyme found only in teeth, chops up amelogenin proteins, which facilitate organised enamel crystal formation. MMP-20 clears the way for hard material to usurp vacated space.

Her team is the first to define the function of an enzyme for preventing protein occlusion inside a crystal, she said.

“MMP-20 is released at a very early stage of enamel formation,” said Prof. Moradian-Oldak, the study’s senior author. “MMP-20 chops up proteins during the crystallisation of enamel. Together with other enzymes, it gets rid of ‘sludge’ so the enamel making cells in the body can add more mineral and make enamel, the hardest bioceramic in the human body.”

new enamel regrowthProf. Moradian-Oldak will marry the MMP-20 discovery with another study published Nov. 2 in the Journal of Biomedical Engineering and Informatics, which concluded an amelogenin-chitosan hydrogel could repair early tooth decay by growing an enamel-like layer that reduces lesions by up to 70 percent.

“Recognizing MMP-20’s function in biomineralization is one of the first steps to learning how dental enamel forms in nature,” said Qichao Ruan, lead author of the hydrogel study and a postdoctoral research associate in the Center for Craniofacial Molecular Biology at USC. “The findings regarding MMP-20 not only help us to further understand the mechanisms of enamel formation but also can be applied in the design of novel biomaterials for future clinical applications in dental restoration or repair.”

Products such as toothpaste and mouthwash containing fluoride and casein phosphopeptide-amorphous calcium phosphate promote remineralization of initial enamel lesions; however, they need to be used regularly and are more of a tire patch than a real solution, Prof. Moradian-Oldak said. It plugs up the problem so people don’t feel pain. The gel, however, fills the cracks and holes with an enamel-like substance.

When tested in an environment that mimics an oral cavity’s biochemical processes, the gel created a robust attachment, eliminating the threat of secondary cavities in the same spot, Ruan said. The gel could be more effective than traditional crowns, whose adhesion weakens over time, he added.

“Besides biocompatibility and biodegradability, the gel has unique antimicrobial and adhesion properties that are important for dental applications,” Ruan said.

USC researchers tested their theory using wild type mice and MMP-20 null mice. The MMP-20 null mice had inconsistent enamel hydroxyapatite crystals that were shorter, wider and thinner than those found in the wild type mice.

Some 31 percent of the enamel nanocrystals area isolated from MMP-20 mice were imperfect, whereas only 10 percent of the area was imperfect in crystals from wild type mice.

In preparation for a possible human study, USC researchers used human molars without any lesions. They sliced teeth into three or four blocks, created artificial tooth decay, then cycled the samples in artificial saliva with pH 4.6, 7.0 and 6.5.

Normal salivary pH is between 6 to 7 but could quickly fluctuate between 5.3 to 7.8 based on food and beverage intake.

A sample of supersaturated calcium and phosphate ions in a remineralization solution produced an enamel-like substance; however, it created a disorganized structure with irregular crystals. In contrast, the hydrogel grew oriented crystals, reducing the depth of the lesions by 50 to 70 percent after seven days of hydrogel application. It is a big improvement over other methods, Ruan said.

“In one study, it was reported that only about 24 percent of tooth decay was recovered after 12 days of pH cycling with sodium fluoride treatment,” he said.

The next step is to alter the gel recipe using MMP-20 to create a stronger enamel-like seal, Prof. Moradian-Oldak said.

“We create a protective cover on enamel,” she said. “We restore the structure of enamel, and it will prevent decay from progressing.”

Previous articleGift empowers oral health research
Next articleFree for a day

LEAVE A REPLY

Please enter your comment!
Please enter your name here